![]() Total body water increases after carbohydrate loading due to binding of glycogen with water. Our results suggest that increase in body water after carbohydrate loading can be detected by BIS and is caused by segment-specific increases in ICW. Segmental BIS showed significant increases in ICW in the legs ( P < 0.05), but not in the arms or trunk. Whole-body BIS showed significant increases in ICW ( P < 0.05), but not in ECW. Muscle glycogen concentration and TBW D2O (39.3 ± 3.2 to 40.2 ± 3.0 kg, P < 0.05) increased significantly 72 h after exercise compared with baseline, respectively. ICW and ECW in the whole body (wrist-to-ankle) and in each body segment (arm, trunk, and leg) were assessed by BIS. Changes in muscle glycogen concentration were measured by 13C-magnetic resonance spectroscopy, and total body water (TBW) was measured by the deuterium dilution technique (TBW D2O). day −1 for 72 h after glycogen depletion cycling exercise.Eight subjects consumed a high-carbohydrate diet containing 12 g carbohydrates Here, we aimed to investigate whether BIS had sufficient sensitivity to detect changes in body water content and to determine segmental water distribution after carbohydrate loading. However, BIS has not been shown to detect changes in body water induced by carbohydrate loading. Bioelectrical impedance spectroscopy (BIS) allows separate assessment of extracellular and intracellular water (ECW and ICW, respectively) in the whole body and each body segment. Body water content increases during carbohydrate loading because 2.7-4-g water binds each 1 g of glycogen. ![]()
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